Journal
ACS SENSORS
Volume 5, Issue 1, Pages 24-28Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b02137
Keywords
electrochemical biosensor; immunosensor; clinical diagnostics; graphene; amyloid precursor protein; antibody variable region; fusion protein
Funding
- JSPS KAKENHI from the Japan Society for the Promotion of Science, Japan [JP18H03851, JP17K06920, JP19H02582]
- JST-Mirai Grant [JPMJMI18D9]
- JST-CREST Grant from Japan Science and Technology Agency, Japan [JPMJCR1SF4]
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Due to its high carrier mobility, graphene is considered a suitable material for use in field-effect transistors. However, its application to immunosensing of small molecules is still elusive. To investigate the potential of graphene field effect transistors (G-FET) as a sensor for small molecules with small or no charge, we applied the open-sandwich immunoassay (OS-IA), which detects low-molecular-weight antigens noncompetitively, to G-FET. Using an antibody variable fragment V-L immobilized on graphene and a hyperacidic region of amyloid precursor protein fused to the other variable fragment V-H, we successfully detected a small antigen peptide consisting of 7 amino acids (BGP-C7), with a more than 100-fold increase in sensitivity compared with that measured by enzyme-linked OS-IA. Furthermore, we succeeded in detecting BGP-C7 in the presence of human serum with similar sensitivity, suggesting its potential application in clinical diagnostics.
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