Journal
AMB EXPRESS
Volume 10, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1186/s13568-020-0956-5
Keywords
Alkanes; Alkylsuccinates; Fumarate addition; Methanogenesis; Oil reservoirs
Categories
Funding
- National Natural Science Foundation of China [41530318, 41403066]
- NSFC/RGC Joint Research Fund [41161160560]
- Fundamental Research Funds for the Central Universities [222201817017, 50321101917017, 22221818014]
- Research Program of State Key Laboratory of Bioreactor Engineering
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In the present study, a methanogenic alkane-degrading (a mixture of C-9 to C(12)n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C-9 to C-12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.
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