Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 6, Issue 3, Pages 1590-1602Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01363
Keywords
exosomes; human umbilical cord mesenchymal stem cells; osteogenesis; hydrogel
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Funding
- National Natural Science Foundation of China [51802350, 51972339, 81703003]
- China Postdoctoral Science Foundation [2017 M623395, 2018 T111143, 2018 M633722]
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The treatment of bone defects has plagued clinicians. Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy. In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro. Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites. Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo. The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration. Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.
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