Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 6, Issue 3, Pages 1500-1509Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01374
Keywords
phosphorous-containing hydrogels; osteogenic differentiation; MAPK signaling pathway
Categories
Funding
- National Key Research and Development Program of China [2018YFA0703000]
- Science and Technology Program of Guangdong Province [2019B010941002, 2017B090911008, 2016A030306018]
- National Nature Science Foundation of China [U1801252, 51603074]
- Science and Technology Program of Guangzhou [201804020060]
- Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory [2018GZR110102001]
- Shenzhen Science and Technology Program [JCYJ20170815153105076, GJHZ20180411143347603]
Ask authors/readers for more resources
Phosphorous-containing biopolymers have been applied to expedite the regeneration of damaged bone tissue by stimulating the function of phosphorous groups in natural bones. However, the underlying mechanism of phosphorous-containing biopolymers in promoting osteogenic differentiation is unclarified. Herein, we synthesized phosphorylated chitosan hydrogels by incorporating phosphocreatine into chitosan molecular chains under mild conditions. The introduction of phosphate groups improved properties of protein adsorption and calcium deposition without affecting the morphology of hydrogels. Our results showed that phosphorylated chitosan hydrogels could not only promote alkaline phosphatase activity and mineralization but also upregulate the expression of osteogenic-related genes and proteins. Meanwhile, application of c-Jun N-terminal kinase inhibitor SP600125 and p38 mitogen-activated protein kinase inhibitor SB203580 repressed the expression of osteogenic-related markers in gene and protein levels. To the best of our knowledge, it is reported for the first time that phosphorous-containing biopolymers promote osteogenic differentiation of osteoblasts via JNK and p38 signaling pathways.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available