Characterization and FPLC Analysis of Garbage Enzyme: Biocatalytic and Antimicrobial Activity

Fermentation of fruit and vegetable peels produces garbage enzyme (GE) which was used to obtain industrially beneficial products. This study was conducted for the characterization of GE and evaluation of its biocatalytic and antimicrobial activity. The maximum activity of amylase, protease and lipase were 1.782 units/mg, 0.050 units/mg and 0.355 units/mg respectively were found in optimum conditions which also showed antioxidant properties. The enzyme solution was separated by Fast Protein Liquid Chromatography (FPLC) and experiments were conducted for antimicrobial activity with the FPLC generated fractions of GE on both gram positive and gram negative bacteria. Inhibition zones were measured from concentration dependent bioassay analysis of lyophilized FPLC fractions. FPLC fraction II offered best zone of inhibition in Escherichia coli, Staphylococcus aureus and in Rhizobium leguminosarum. Molecular weight of above fraction was determined by SDS-PAGE technique. Significant research findings of this research work will help in developing newer potential commercializable antimicrobial agents from GE generated from fruit and vegetable waste. [GRAPHICS] .

Automated summary

This study characterized garbage enzyme (GE) and evaluated its biocatalytic and antimicrobial activity. The results showed that GE exhibited antioxidant properties and effective inhibition against both gram positive and gram negative bacteria, particularly in E. coli, S. aureus, and R. leguminosarum. The research findings suggest the potential for developing commercializable antimicrobial agents from GE derived from fruit and vegetable waste.