4.6 Article

The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia

Journal

PEERJ
Volume 8, Issue -, Pages -

Publisher

PEERJ INC
DOI: 10.7717/peerj.8664

Keywords

Gut microbiota; Hyperuricemia; Correlation; Change; Gut microbiota disorder; Fecal microbiota transplantation

Funding

  1. National Natural Science Foundation of China [81671625, 81100554, 81901575]
  2. Young and Middle-Aged Scientists Research Awards Fund of Shandong Province [BS2012YY003]
  3. Scientific and Technical Development Project of Department of Health of Shandong Province [2011QZ007, 2016WS0259]
  4. Shandong Province Natural Science Fund Project [ZR2018PH010, ZR2014HM015]
  5. Project of Shandong Province Higher Educational Science and Technology Program [J14LK11]
  6. Scientific and Technical Development Project of Qingdao [12-1-4-20jc, 2012-1-3-2-(1)-nsh, 2013-13-008-YY, 2014-1-72, 17-3-3-15-nsh]

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Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target.

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