Journal
NANOMATERIALS
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/nano10020281
Keywords
gold nanocluster; 6-aza-2-thio-thymine; protein; fluorescence
Categories
Funding
- National Natural Science Foundation of China [21675024]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Science and Technology Project of Fujian Province [2018L3008]
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This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).
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