Journal
NANOMATERIALS
Volume 10, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/nano10020219
Keywords
F. graminearum; nanoparticles; SEM; FTIR; antifungal activity; mycotoxins
Categories
Funding
- National Key Research and Development Program of China [2017YFD0201104, 2018YFD0300900]
- National Natural Science Foundation of China [31872017, 31571971, 31371904, 31801787, 31901925]
- Zhejiang Provincial Natural Science Foundation of China [LZ19C140002]
- Zhejiang Provincial Project [2017C02002, 2019C02006, 2020C02006]
- Shanghai Agriculture Applied Technology Development Program [2019-02-08-00-08-F01150]
- Fundamental Research Funds for the Central Universities, Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University
- Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control
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Nanoparticles are expected to play a vital role in the management of future plant diseases, and they are expected to provide an environmentally friendly alternative to traditional synthetic fungicides. In the present study, silver nanoparticles (AgNPs) were green synthesized through the mediation by using the endophytic bacterium Pseudomonas poae strain CO, which was isolated from garlic plants (Allium sativum). Following a confirmation analysis that used UV-Vis, we examined the in vitro antifungal activity of the biosynthesized AgNPs with the size of 19.8-44.9 nm, which showed strong inhibition in the mycelium growth, spore germination, the length of the germ tubes, and the mycotoxin production of the wheat Fusarium head blight pathogen Fusarium graminearum. Furthermore, the microscopic examination showed that the morphological of mycelia had deformities and collapsed when treated with AgNPs, causing DNA and proteins to leak outside cells. The biosynthesized AgNPs with strong antifungal activity were further characterized based on analyses of X-ray diffraction, transmission electron microscopy, scanning electron microscopy, EDS profiles, and Fourier transform infrared spectroscopy. Overall, the results from this study clearly indicate that the biosynthesized AgNPs may have a great potential in protecting wheat from fungal infection.
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