Journal
MICROBIOLOGYOPEN
Volume 9, Issue 3, Pages -Publisher
WILEY
DOI: 10.1002/mbo3.979
Keywords
baculovirus expression system; coexpression; reBmBac system; recombinant chicken interferon
Categories
Funding
- National Key Research and Development Program of China [2016YFD0500108, 2017YFD0500706]
- National Natural Science Foundation of China [31670156, 31872430]
Ask authors/readers for more resources
The Bombyx mori nucleopolyhedrovirus (BmNPV) baculovirus expression system (BES) is a eukaryotic expression system. It possesses great capability for post-translation modification in expression of foreign proteins. With the counterselection cassette rpsL-neo and phage lambda-Red recombinase, the defective-rescue BmNPV BES reBmBac can be employed for efficient heterologous multigene coexpression at different gene sites in one baculovirus genome. In the present study, a recombinant baculovirus, reBm-C alpha gamma, carrying two types of chicken interferon (IFN) genes (chIFN-alpha and chIFN-gamma) was constructed using the reBmBac system. The chIFN-alpha and chIFN-gamma genes were inserted into the same baculovirus genome at the polyhedron and p10 gene sites, respectively. The recombinant baculovirus was capable of coexpressing both chIFN-alpha and chIFN-gamma. The expression levels of the two types of IFN in the coexpression product were exponentially high, at approximately 1.7 and 2.5 times higher, respectively, than those in the corresponding single-expression products. The increase in expression level corresponds to replacement of the nonessential p10 gene in the reBm-C alpha gamma recombinant baculovirus. This coexpression of recombinant chicken IFNs showed superior antiviral activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available