Journal
FRONTIERS IN PHARMACOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2020.00021
Keywords
alginate; seleno-polymannuronate; anti-apoptosis; neuroprotection; N2a-sw cells
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Funding
- National Key R&D Program of China [2018YFD0901106]
- National Natural Science Foundation of China [31871734, 21877081]
- Guangdong Natural Science Foundation [2018A0303130054, 2016A030313052, 2018A030313507]
- Science and Technology Innovation Commission of Shenzhen [JCYJ20170818143107733, JCYJ20180507182405562, JCYJ20180305124211995, JCYJ20180305125619343]
- Guangdong Natural Science Foundation for Major Cultivation Project [2018B030336001]
- State Oceanic Administration 13th Five-Year Marine Special Fund for Demonstration City, Shenzhen Basic Research Project (Subject Layout Project)
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Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 mu g/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of A beta(1-42) oligomer in vitro and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect.
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