Journal
SUSTAINABILITY
Volume 12, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/su12010431
Keywords
organofluorine compound; fluorinase; 5 '-fluorodeoxy adenosine; biomineralization; immobilization; fluoridated hydroxyapatite nanoflowers
Funding
- National Natural Science Foundation of China [21576062, 81730108]
- Natural Science Foundation of Zhejiang Province [LY18B060009, LY15B060011]
- National Innovation and Entrepreneurship Training Program for Undergraduate [201810346008]
- Technology Research and Development Program of Hangzhou [20191203B09]
- National innovation and entrepreneurship project for College Students [201810346008, 201910346044]
- Scientific Research Innovation Fund for Graduate Students of Hangzhou Normal University, Star and light Project for Talent Students in Hangzhou Normal University
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To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the enzyme@fluoridated hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained fluorinase@FHAp-NFs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5 '-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 degrees C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5 '-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds.
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