Journal
MICROMACHINES
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/mi11030237
Keywords
three-dimensional bioprinting; vascularized channels; extrusion-based printing; drop-based printing; hydrogel; perfusion
Categories
Funding
- Natural Science Foundation of Jiangsu Province [BK20190712]
- Key Technology RD Program of Jiangsu Province [BE2018010]
- University Natural Science Research Project of Jiangsu Province [19KJD460005]
- Open fund of Jiangsu Key Laboratory of 3D Printing Equipment and Application Technology [2018KFKT02]
- Nantong Key Laboratory of 3D printing technology and Application [CP12016002]
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Three-dimensional bioprinting has emerged as one of the manufacturing approaches that could potentially fabricate vascularized channels, which is helpful to culture tissues in vitro. In this paper, we report a novel approach to fabricate 3D perfusable channels by using the combination of extrusion and inkjet techniques in an integrated manufacture process. To achieve this, firstly we investigate the theoretical model to analyze influencing factors of structural dimensions of the printed parts like the printing speed, pressure, dispensing time, and voltage. In the experiment, photocurable hydrogel was printed to form a self-supporting structure with internal channel grooves. When the desired height of hydrogel was reached, the dual print-head was switched to the piezoelectric nozzle immediately, and the sacrificial material was printed by the changed nozzle on the printed hydrogel layer. Then, the extrusion nozzle was switched to print the next hydrogel layer. Once the printing of the internal construct was finished, hydrogel was extruded to wrap the entire structure, and the construct was immersed in a CaCl2 solution to crosslink. After that, the channel was formed by removing the sacrificial material. This approach can potentially provide a strategy for fabricating 3D vascularized channels and advance the development of culturing thick tissues in vitro.
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