Journal
BIOMED RESEARCH INTERNATIONAL
Volume 2020, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2020/5393041
Keywords
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [402481/2016-0]
- CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
- FundacAo de Amparo a Pesquisa Carlos Chagas Filho (FAPERJ)
- FundacAo do Cancer
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Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 mu M of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate ((32)Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, rho ACC beta, pAMPK alpha, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of rho ACC beta (3.3-fold) and AMPK alpha/rho AMPK alpha (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of rho ACC beta (111.8-fold) and AMPK alpha/rho AMPK alpha (1.2 fold). Our results show that Resv modified PL and saturated and unsaturated FA especially in MDA-MB-231 cells, and open new perspectives to the understanding of the reported anticancer effect of Resv on these cells.
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