Journal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 46, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.algal.2019.101688
Keywords
Chlorophyllide sters; Internal Standard; Pigment analysis; Chromatography
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Funding
- Spanish Ministerio de Economia y Competitividad (MINECO)
- FEDER [CTM2012-32181, BES-2013065752]
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In this article the chlorophyllase activity of Dunaliella sauna has been employed to generate different chlorophyllide a and b esters that could potentially be used as internal standards in pigment analysis. Chlorophyllide a (8'-hydroxyoctyl) ester was selected due its adequate chromatographic and spectral properties and was fully characterized by UV-Vis, ESI-MS and NMR. An easy room temperature procedure for its synthesis is described. Attachment
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