Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 8, Issue 11, Pages 4481-4488Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.9b07631
Keywords
boronic acid; colorimetric detection; hierarchical pores; metal-organic frameworks; nanozymes
Categories
Funding
- National Natural Science Foundation of China [21765002]
- Guangxi Natural Science Foundation of China [2017GXNSFDA198044]
- BAGUI Scholar Program
Ask authors/readers for more resources
Metal-organic frameworks (MOFs) with uniform porosity and large surface areas can function as carriers of immobilized enzymes. However, drawbacks including long response times or enzyme leakage have hindered their applicability. In this study, a boronic acid-functionalized hierarchically porous MIL-88B (HP-MIL-88B-BA) was prepared as an efficient immobilization matrix for glucose oxidase (GOx). HP-MIL-88B-BA features a hierarchical, porous structure with sufficient recognition sites that facilitate GOx immobilization and prevent enzyme leakage. This hierarchical porosity increased the substrate mass transfer efficiency, thereby reducing the response time. Moreover, HP-MIL-88BBA can be used as a horseradish peroxidase mimic. In this manner, an integrated nanozyme was constructed for the rapid one-step detection of glucose. GOx@HP-MIL-88B-BA exhibited a rapid response to glucose (10 min) and displayed a good linear relationship from 2 to 100 mu M with a detection limit of 0.98 mu M. This novel methodology provides a straightforward, rapid, and efficient strategy for integrated nanozyme synthesis for biosensing purposes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available