Journal
POLYMERS
Volume 12, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/polym12010191
Keywords
benzenesulfonic acid pretreatment; components removal; lignin deposition; enzymatic hydrolysis; lignin-shielding agent
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Funding
- Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology [KL201911]
- National Natural Science Foundation of China [31870559, 31901262]
- Fujian Provincial Department of Science and Technology, and Education [2018J01590, 2019J01387, FBJG20180196]
- Scientific and Technological Innovation Funding of Fujian Agriculture and Forestry University [CXZX2017034, CXZX2017293, CXZX2017374, CXZX2018004]
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A hydrotrope-based pretreatment, benzenesulfonic acid (BA) pretreatment, was used to fractionate bamboo in this work. With optimized content (80 wt %) of BA in pretreatment liquor, about 90% of lignin and hemicellulose could be removed from bamboo under mild conditions (95 degrees C, 30 min or 80 degrees C, 60 min). The potential accessibility of BA pretreated substrate to cellulase was thus significantly improved and was also found to be much higher than those of acidic ethanol and dilute acid pretreatments. But the deposition of lignin on the surface of solid substrates, especially the BA pretreated substrate, was also observed, which showed a negative effect on the enzymatic hydrolysis efficiency. The addition of inexpensive soy protein, a bio-based lignin-shielding agent, could readily overcome this negative effect, leading the increase of enzymatic conversion of cellulose in BA pretreated substrate from 37% to 92% at a low cellulase loading of 4 FPU/g glucan. As compared to acidic ethanol and dilute acid pretreatments, the combination of BA pretreatment and soy protein could not only stably improve the efficiency of non-cellulose components removal, but also could significantly reduce the loading of cellulase.
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