Journal
PLOS BIOLOGY
Volume 17, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.3000549
Keywords
-
Categories
Funding
- Wellcome Trust [101799/Z/13/Z, 097108/Z/11/Z, 099812/Z/12/Z]
- Australian Academy of Science [1016848]
- National Health and Medical Research Council of Australia [APP1163814]
- Wellcome Trust [097108/Z/11/Z, 099812/Z/12/Z] Funding Source: Wellcome Trust
- MRC [G9722488, G19/31] Funding Source: UKRI
Ask authors/readers for more resources
Dose-response experiments are a mainstay of receptor biology studies and can reveal valuable insights into receptor function. Such studies of receptors that bind cell surface ligands are currently limited by the difficulty in manipulating the surface density of ligands at a cell-cell interface. Here, we describe a generic cell surface ligand system that allows precise manipulation of cell surface ligand densities over several orders of magnitude. These densities are robustly quantifiable, a major advance over previous studies. We validate the system for a range of immunoreceptors, including the T-cell receptor (TCR), and show that this generic ligand stimulates via the TCR at a similar surface density as its native ligand. We also extend our work to the activation of chimeric antigen receptors. This novel system allows the effect of varying the surface density, valency, dimensions, and affinity of the ligand to be investigated. It can be readily broadened to other receptor-cell surface ligand interactions and will facilitate investigation into the activation of, and signal integration between, cell surface receptors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available