Journal
CELL REPORTS
Volume 30, Issue 4, Pages 1129-+Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2019.12.066
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Funding
- Canadian Institutes of Health Research (CIHR) [MT-14429, MOP-82906, FDN-143338, MOP-142333, PJT-152988]
- National Natural Science Foundation of China [NSFC-31870863]
- Cincinnati Children's Research Foundation [DA038017, TR001425]
- National Institutes of Health [DA038017, TR001425]
- CIHR
- Japan Society for the Promotion of Science (JSPS)
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Plasma membrane damage and cell death during processes such as necroptosis and apoptosis result from cues originating intracellularly. However, death caused by pore-forming agents, like bacterial toxins or complement, is due to direct external injury to the plasma membrane. To prevent death, the plasma membrane has an intrinsic repair ability. Here, we found that repair triggered by pore-forming agents involved TMEM16F, a calcium-activated lipid scramblase also mutated in Scott's syndrome. Upon pore formation and the subsequent influx of intracellular calcium, TMEM16F induced rapid lipid scrambling in the plasma membrane. This response was accompanied by membrane blebbing, extracellular vesicle release, preserved membrane integrity, and increased cell viability. TMEM16F-deficient mice exhibited compromised control of infection by Listeria monocytogenes associated with a greater sensitivity of neutrophils to the pore-forming Listeria toxin listeriolysin O (LLO). Thus, the lipid scramblase TMEM16F is critical for plasma membrane repair after injury by pore-forming agents.
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