Journal
ANIMAL BIODIVERSITY AND CONSERVATION
Volume 43, Issue 1, Pages 67-+Publisher
MUSEU DE CIENCIES NATURALS-ZOOLOGIA
DOI: 10.32800/abc.2020.43.0067
Keywords
DNA isolation; DNA concentration; DNA purity; Price of extraction kit
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Funding
- Ministry of Culture of the Czech Republic: project NAKI II [DG-16P02B038]
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Comparison of 12 DNA extraction kits for vertebrate samples. Obtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column-based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick (R) Genomic DNA Purification Kit (Genomed) and the NucleoSpin (R) Tissue (Macherey-Nagel). Nevertheless, it is highly recommended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project.
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