Journal
ACS SYNTHETIC BIOLOGY
Volume 9, Issue 1, Pages 63-75Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.9b00334
Keywords
cyanobacteria; indolactam; secondary metabolites; heterologous expression
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Funding
- Oregon State University NMR Facility
- SOU Biotechnology Research Center
- College of Pharmacy, Oregon State University
- Medical Research Foundation of Oregon [1415]
- AREA award grant from the National Institutes of Health [1R15GM117541]
- Southern Oregon University
- National Institutes of Health, HEI Grant [1S100D018518]
- M. J. Murdock Charitable Trust Grant [2014162]
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Cyanobacteria are prolific producers of natural products, and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters, and here we present the use of Anabaena sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native Anabaena 7120 plasmid pCC7120 zeta and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by coconjugation.
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