Journal
REVIEWS IN AQUACULTURE
Volume 12, Issue 3, Pages 1928-1943Publisher
WILEY
DOI: 10.1111/raq.12417
Keywords
enzyme unit; fish; pepsin-like acid protease; protocol; stomach
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Funding
- CYTED Network LARVAplus [117RT0521]
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Assessing the activity of acid pepsin-like protease is a common procedure in many biological, physiological and nutritional studies in gastric fish species. After reviewing the available literature on fish digestive acid proteases (stomach acid pepsin-like proteases), especially those species of relevance for aquaculture purposes, published during a time span of ten years (2008-2018), authors detected some possible methodological and/or interpretative inconsistencies in this kind of studies when quantifying pepsin-like enzymes. Thus, we concluded that special attention should be paid on the following issues when assessing digestive acid protease activity: (i) the proper preparation of stomach extracts, (ii) the use of the proper reaction conditions at the normal range of physiological pH and temperature values, and (iii) the proper quantification of the number of peptide bonds hydrolyzed for acid protease unit calculation. The implementation of these recommendations, and the proposal of more suitable (previously tested) methodological steps, will promote the standardization of acid pepsin-like protease analytical procedures, as well as improve the reliability of comparative studies dealing with this proteolytic enzyme between different fish species of aquacultural importance.
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