Journal
EPIGENOMICS
Volume 12, Issue 4, Pages 289-302Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2019-0218
Keywords
bioinformatics; circRNA-miRNA-mRNA network; circular RNA; hADSCs; osteogenic differentiation
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Aim: Circular RNAs (circRNAs) are essential for stem cell differentiation. This study aimed to investigate their exact mechanism of action in human adipose-derived stem cell (hADSC) osteogenesis. Materials & methods: Isolated hADSCs were cultured in growth medium or osteogenic medium, then total RNA was extracted for circRNA microarray, hierarchical cluster, gene ontology, regulating pathway and circRNA-miRNA-mRNA network analyses. Results: A total of 171 circRNAs were upregulated and 119 were down-regulated in induced groups compared with those in noninduced groups. Eight circRNAs, 40 miRNAs and 342 mRNAs were selected to construct a competing circRNA-miRNA-mRNA network. Conclusion: These findings may provide novel insight into altered and specific circRNAs that might function as competing endogenous RNAs in hADSCs during osteogenic differentiation.
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