Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 19, Issue 2, Pages 1288-1296Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2019.8330
Keywords
microRNA-155; forkhead box protein O3a; rheumatoid arthritis; inflammation
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Funding
- Science Foundation of Inner Mongolia [2016MS (LH) 0808]
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The present study aimed to explore the expression and effects of microRNA (miR)-155 in synovial fibroblasts of patients with rheumatoid arthritis (RA). A total of 89 synovial tissues from RA patients and 49 control synovial tissues were collected, and the levels of miR-155 were measured by reverse transcription quantitative-PCR and western blotting. Fibroblast-like synoviocytes (FLS) were isolated from synovial tissues from the control group and were used to evaluate the roles of miR-155 and forkhead box protein O3a (FOXO3a). MTT assay was used to measure the proliferation of FLS. The expression of miR-155 in RA synovial tissues was significantly higher than that in the control group, but the expression of FOXO3a was significantly lower. In RA synovial tissues, miR-155 expression was negatively correlated with FOXO3a expression, but was positively correlated with the release of inflammatory cytokines interleukin (IL)-1 beta, IL-6 and tumor necrosis factor-alpha (TNF-alpha). A dual-luciferase reporter system showed that miR-155 inhibited the expression of FOXO3a in FLS cells. miR-155 also promoted secretion of the inflammatory cytokines IL-1 beta, IL-6 and TNF-alpha by FLS and proliferation of these cells by targeting FOXO3a.
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