Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 59-65Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.06.033
Keywords
Pd-Fe3O4-GS; SiO2; Sandwich-type electrochemical immunosensor; Human immunoglobulin G; Tumor markers
Categories
Funding
- National Natural Science Foundation of China [21175057, 21375047, 21377046, 21405059]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
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An ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy was developed for the quantitative determination of human immunoglobulin G (IgG). Pd nanocubes functionalized magnetic graphene sheet (Pd-Fe3O4-GS) was employed as the matrix to immobilize the primary antibodies (Ab(1)). Owing to the synergetic effect between Pd nanocubes and magnetic graphene sheet (Fe3O4-GS), Pd-Fe3O4-GS can provide an obviously increasing electrochemical signal by electrochemical catalysis towards hydrogen peroxide (H2O2). Silicon dioxide (SiO2) was functionalized as the label to conjugate with the secondary antibodies (Ab(2)). Due to the larger steric hindrance of the obtained conjugate (SiO2@Ab(2)), the sensitive decrease of the electrochemical signal can be achieved after the specific recognition between antibodies and antigens. In this sense, this proposed immunosensor can achieve a high sensitivity, especially in the presence of low concentrations of IgG. Under optimum conditions, the proposed immunosensor offered an ultrasensitive and specific determination of IgG down to 3.2 fg/mL. This immunoassay method would open up a new promising platform to detect various tumor markers at ultralow levels for early diagnoses of different cancers. (C) 2015 Elsevier B.V. All rights reserved.
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