Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 64, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jff.2019.103677
Keywords
Homogeneous polysaccharide; Colon cancer; Cell cycle; Proliferation; Phospho-AKT; MAPK; Hawthorn polysaccharide; Apoptosis
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Funding
- National Natural Science Foundation of China [31371815]
- Natural Sciences Foundation of Hebei Province [15227106D]
- USDA HATCH projects [ILLU-698323, ILLU-698369]
- ACES International Joint Research Program in the University of Illinois at Urbana-Champaign
- Multi-State project [W4122]
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This study demonstrated that a homogeneous polysaccharide (HPS) extracted from Hawthorn exerted anticancer effects on colon cancer cells. Human colon cancer cell line HCT116 were treated with 125-1000 mu g/mL HPS for 12 h and subsequent analysis was performed on proliferation and signaling pathways. HPS significantly inhibited proliferation of HCT116 cells when analyzed using WST-1 assay and immunofluorescence staining of Ki-67. Flow cytometric studies revealed that HPS could arrest the cell cycle in the S and G2/M phases and increase the rate of apoptosis. HPS downregulated the expression of Cyclin A1/D1/E1 and CDK-1/2. HPS induced apoptosis on HCT116 cells through upregulation of caspase3, 7, 8, 9 and Fas, FADD, TNF-R1, TRADD, upregulation of caspase3 protein was also observed. HPS has an anti-cancer potential in the treatment of human colon cancer which makes it a potential candidate in functional foods for cancer patients.
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