Journal
BIOSENSORS & BIOELECTRONICS
Volume 67, Issue -, Pages 595-600Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.09.059
Keywords
Graphene quantum dots (GQDs); Gold nanoparticles (AuNPs); Fluorescence resonance energy transfer (FRET); Foodborne bacteria
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Funding
- Hong Kong Research Council General Research Grant [PolyU5305/11E]
- Hong Kong Ph.D Fellowship Scheme Fund [HKPF10-13386/ PolyU RUY5]
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In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor. (C) 2014 Elsevier B.V. All rights reserved.
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