Journal
BIOSENSORS & BIOELECTRONICS
Volume 63, Issue -, Pages 450-457Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.07.077
Keywords
Lab-on-paper; Peroxydisulfate; AuPd alloy nanoparticles; Cell; Electrochemiluminescence; Cancer diagnosis
Categories
Funding
- National Natural Science Foundation of China [21175058, 21277058, 21207048]
- Natural Science Foundation of Shandong Province, China [ZR2012BZ002]
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The detection of cancer cells is important and fundamental for cancer diagnosis and therapy, which has attracted considerable interest recently. Although traditional cyto-sensors have been widely explored due to their high sensitivity and selectivity, it is still a challenge to develop a low-cost, portable, disposable, fast, and easy-to-use cancer cell detection method for applying in the field of cancer diagnosis and therapy. Herein, to address these challenges, we developed a microfluidic paper-based electrochemiluminescence origami cyto-device (mu-PECLOC), in which aptamers modified 3D macroporous Aupaper electrodes were employed as the working electrodes and efficient platforms for the specific cancer cells capture. Owing to the effective disproportionation of hydrogen peroxide and specific recognition of mannose on cell surface, concanavalin-A conjugated porous AuPd alloy nanoparticles were introduced into this mu-PECLOC as the catalytically promoted nanolabels for peroxydisulfate ECL system. Under the optimal conditions, the proposed mu-PECLOC exhibited excellent analytical performance with good stability, reproducibility, and accuracy, towards the cyto-sensing of four types of cancer cells indicating the potential applications to facilitate effective and multiple early cancer diagnosis and clinical treatment. (C) 2014 Elsevier B.V. All rights reserved.
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