Simultaneous Ammonium oxidation denitrifying (SAD) in an innovative three-stage process for energy-efficient mature landfill leachate treatment with external sludge reduction

High-loaded ammonia and low-strength organics mature landfill leachate is not effectively treated by conventional biological processes. Herein, an innovative solution was proposed using a three-stage Simultaneous Ammonium oxidation Denitrifying (SAD) process. Firstly, ammonia (1760 +/- 126 mg N/L) in wastewater was oxidized to nitrite in a partial nitrification sequencing batch reactor (PN-SBR). Next, 93% PN-SBR effluent and concentrated external waste activated sludge (WAS; MISS = 23057 +/- 6014 mg/L) were introduced to an anoxic reactor for integrated fermentation and denitrification (IFD-SBR). Finally, ammonia (101.4 +/- 13.8 mg N/L) released by fermentation in the IFD-SBR and residual 7% nitrite in the PNSBR were removed through the anaerobic ammonium oxidation (anammox) process in the SAD up-flow anaerobic sludge bed (SAD-UASB). In addition, NO3--N generation during the anammox process could be reduced to nitrite by partial denitrification (PD) and reused as substrate for anammox. A satisfactory total nitrogen (TN) removal efficiency (98.3%), external sludge reduction rate (2.5 kg/m(3) d) and effluent TN concentration (16.7 mg/L) were achieved after long-term operation (280 days). The IFD-SBR and SADUASB contributed to 81.9% and 123% nitrogen removal, respectively. Microbial analysis showed that anammox bacteria (1.5% Candidatus Brocadia) cooperated well with partial denitrifying bacteria (4.3% Thauera) in SAD-UASB, and average nitrogen removal contribution were 83.1% during significant stability of anammox and 9.2% during the denitrification process, respectively. The three-stage SAD process provides an environmental and economic approach for landfill leachate treatment since it has the advantage of 25.4% less oxygen, 100% organic matter savings and 47.9% less external sludge than traditional biological processes. (C) 2019 Elsevier Ltd. All rights reserved.