Journal
BIOSENSORS & BIOELECTRONICS
Volume 74, Issue -, Pages 98-103Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.069
Keywords
Vascular endothelial growth factor; Aptamer; Exonuclease; Electrochemiluminescence; Signal amplification
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Funding
- National Natural Science Foundation of China [21175067, 21273117, 21375063, 21335004, 21405083]
- Natural Science Foundation of the Jiangsu Higher Education Institutions [13KJB150024]
- Programs for Outstanding Innovation Research Team of Universities in Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The expression profile of vascular endothelial growth factor (VEGF) is highly correlated with the occurrence and development of cancer. This work reports an electrochemiluminescence (ECL) approach for highly sensitive detection of VEGF(165). This approach comprises aptamer-target recognition, T7 exonuclease (17 Exo)-assisted cycling signal amplification and efficient quenching of ECL of CdS:Eu nanocrystals (NCs) by using DNAzyme. In this assay, CdS:Eu NCs were used as the ECL substrate, A guanine (G)-rich single-stranded DNA (ssDNA) sequence and VEGF(165) aptamer were co-immobilized on the surface of the CdS:Eu NCs modified glassy carbon electrode. After recognition and binding to VEGF(165), the aptamer moved away from the electrode surface and induced the proposed cyclic cleavage of the target DNA with T7 Exo. A large amount of G-rich ssDNA was released on the CdS:Eu film and folded into G-quadruplex/hemin DNAzyme in the presence of hemin and K+, consequently decreasing the ECL intensity of CdS:Eu. A good linearity was obtained for VEGF(165) detection within the range of 1 pM to 20 nM with a detection limit of 0.2 pM. This assay could be a universal and promising protocol for detection of various biomarkers for early clinical diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
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