Journal
CANCER LETTERS
Volume 373, Issue 1, Pages 109-118Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2016.01.030
Keywords
CRISPR-Cas9; Sarcoma; Cancer modeling; Gene therapy
Categories
Funding
- Gattegno and Wechsler funds
- Kenneth Stanton Fund
- Jennifer Hunter Yates Foundation
- Sarcoma Foundation of America (SFA)
- National Cancer Institute (NCI)/National Institutes of Health (NIH) [U01, CA 151452-01]
- Sarcoma SPORE/NIH [5U54 CA168512-02]
- Academic Enrichment Fund of MGH Orthopaedics
- China Scholarship Council
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Sarcomas include some of the most aggressive tumors and typically respond poorly to chemotherapy. In recent years, specific gene fusion/mutations and gene over-expression/activation have been shown to drive sarcoma pathogenesis and development. These emerging genomic alterations may provide targets for novel therapeutic strategies and have the potential to transform sarcoma patient care. The RNA guided nuclease CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein-9 nuclease) is a convenient and versatile platform for site-specific genome editing and epigenome targeted modulation. Given that sarcoma is believed to develop as a result of genetic alterations in mesenchymal progenitor/stem cells, CRISPR-Cas9 genome editing technologies hold extensive application potentials in sarcoma models and therapies. We review the development and mechanisms of the CRISPR-Cas9 system in genome editing and introduce its application in sarcoma research and potential therapy in clinic. Additionally, we propose future directions and discuss the challenges faced with these applications, providing concise and enlightening information for readers interested in this area. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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