4.8 Article

BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury(II) ions

Journal

BIOSENSORS & BIOELECTRONICS
Volume 66, Issue -, Pages 251-258

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.11.032

Keywords

Protein; Platinum; Nanoparticle; Nanozyme; Hg2+ detection

Funding

  1. National Natural Science Foundation of China [21176174, 21206107]
  2. Program for Changjiang Scholars and Innovative Research Team in University [IRT1161]

Ask authors/readers for more resources

Bovine serum albumin (BSA) is chosen as the nucleation templates to synthesize Pt-based peroxidase nanomimetics with the average diameter of 2.0 nm. The efficient Pt nanozymes consist of 57% Pt and 43% Pt2+, which possess highly peroxidase-like activity with the K-m values of 0.119 mM and 41.8 mM toward 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), respectively. Interestingly, Hg2+ is able to down-regulate the enzymatic activity of Pt nanoparticles, mainly through the interactions between Hg2+ and Pt. It is the first report to explore a colorimetric Hg2+ sensing system on the basis of peroxidase mimicking activities of Pt nanoparticles. One of our most intriguing results is that BSA-stabilized Pt nanozymes demonstrate the ability to sense Hg2+ ions in aqueous solution without significant interference from other metal ions. The Hg2+ detection limit of 7.2 nM is achieved with a linear response range of 0-120 nM, and the developed sensing system is potentially applicable for quantitative determination of Hg2+ in drinking water. (C) 2014 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available