Journal
CANCER LETTERS
Volume 376, Issue 2, Pages 367-376Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2016.04.016
Keywords
Wilms tumor gene 1 (WT1); TP53; KIT; Testicular germ cell tumors; Next generation sequencing
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Funding
- Internal Grant Agency of the Czech Ministry of Health [IGA NT/12414-5]
- Charles University Grant Agency [GAUK 56413]
- Czech Ministry of Education, Youth and Sports [NPU I LO1604]
- University Hospital Motol, Prague [00064203FNM]
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Purpose: Wilms tumor gene 1 (WT1), a zinc-finger transcription factor essential for testis development and function, along with other genes, was investigated for their role in the pathogenesis of testicular germ cell tumors (TGCT). Methods: In total, 284 TGCT and 100 control samples were investigated, including qPCR for WT1 expression and BRAF mutation, p53 immunohistochemistry detection, and massively parallel amplicon sequencing. Results: WTI was significantly (p < 0.0001) under-expressed in TGCT, with an increased ratio of exon 5-lacking isoforms, reaching low levels in chemo-naive relapsed TGCT patients vs. high levels in chemotherapy-pretreated relapsed patients. BRAE V600E mutation was identified in 1% of patients only. p53 protein was lowly expressed in TGCT metastases compared to the matched primary tumors. Of 9 selected TGCT-linked genes, RAS/BRAF and WTI mutations were frequent while significant TP53 and KIT variants were not detected (p = 0.0003). Conclusions: WT1 has been identified as a novel factor involved in TGCT pathogenesis, with a potential prognostic impact. Distinct biologic nature of the two types of relapses occurring in TGCT has been demonstrated. Differential mutation rate of the key TGCT-related genes has been documented. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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