Fluorescent probes with multiple channels for simultaneous detection of Cys, Hcy, GSH, and H2S

Simultaneous detection of two or more biothiols by using fluorescent methods is in particular importance for biological research and disease diagnosis. Because (1) the variation of one biothiol level under specific cellular metabolism may lead to the change of another biothiol, and (2) several diseases such as Alzheimer's disease associate with joint action from two or more biothiols. In this paper, we provide an overview of the fluorescent probes with multiple channels that have been applied to simultaneous detection of biothiols. The present review highlights the basic characteristics of probes with multi-channel in the respect of design principle, photophysical process, sensing mechanism, sensing performance, and bioapplications. This review aims to offer a comprehensive summary for researchers to inspire new probe design, and also push forward the advancement of simultaneous detection of biothiols based on multi-channel probes. (c) 2019 Elsevier B.V. All rights reserved.