Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 285-291Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.089
Keywords
Peroxynitrite sensor; ONOO- sensor; Reactive oxygen species probe; Fluorescent chemosensor; Cell imaging
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Funding
- National Research Foundation of Korea (NRF) - Korean Government (Ministry of Science, ICT and Future Planning) [2012R1A3A2048814]
- NRF - Ministry of Education [2013R1A1A2008511]
- NRF - Ministry of Science, ICT and Future Planning [NRF-2013M3A9D5072551]
- National Research Foundation of Korea [2013R1A1A2008511] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7 nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology. (C) 2014 Elsevier B.V. All rights reserved.
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