Journal
THERIOGENOLOGY
Volume 141, Issue -, Pages 35-40Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2019.09.010
Keywords
Mogroside V; Oocyte in vitro maturation; ROS; Mitochondria; Embryo development
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Funding
- Scientific Research Foundation of Guangxi University [XTZ170099]
- Guangxi Natural Science Foundation Program [2018GXNSFAA138125]
- One-Hundred Talent Program of Guangxi
- Innovation Project of Guangxi Graduate Education
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Oocyte in vitro maturation (IVM) plays a pivotal role in in vitro embryo production. However, the efficiency of IVM is still low and needs to be further improved. In the present study, we evaluated the beneficial effects of mogroside V, an extract derived from Siraitia grosvenorii, on oocyte IVM. Porcine cumulus-oocyte complexes were cultured in IVM medium supplemented or not supplemented with mogroside V for 40 h. We found that mogroside V supplementation increased the percentage of oocyte first polar body extrusion and improved subsequent blastocyst formation after parthenogenetic activation. Furthermore, mogroside V reduced the levels of reactive oxygen species (ROS) and increased the mRNA expression of oxidative stress-related genes (SOD, CAT and SIRT1). Moreover, mogroside V supplementation enhanced the mitochondrial content, mtDNA copy number, mitochondrial membrane potential (Delta Psi m), ATP generation, and the relative mRNA expression of mitochondria-related genes (PGC-1 alpha and TFAM). In summary, our findings demonstrate that mogroside V supplementation reduces intracellular ROS levels and enhances mitochondrial function to promote porcine oocyte IVM. (C) 2019 Elsevier Inc. All rights reserved.
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