Development of a dispersive liquid-liquid microextraction method based on a ternary deep eutectic solvent as chelating agent and extraction solvent for preconcentration of heavy metals from milk samples

A simple, green, rapid, and efficient ligandless dispersive liquid-liquid microextraction procedure based on solidification of a ternary deep eutectic solvent has been proposed for the extraction of trace amounts of Cd(II), Cu (II), and Pb(II) ions in milk samples prior to their determination by flame atomic absorption spectrometry. Initially, a suitable mole ratio of sorbitol, menthol, and mandelic acid are mixed in a test tube, and after heating, a deep eutectic solvent with low density compared to water is formed. The synthesized solvent is used as a chelating agent and an extraction solvent in the extraction of some heavy metal ions from milk samples. The influence of several effective parameters on the responses and extraction recoveries of the analytes was investigated. Under the optimum experimental conditions, the linear ranges were in the ranges of 1.0-40 mu g L-1 for Cd(II) and pB(II), and 1.0-25 mu g L-1 for Cu(II). The obtained relative standard deviations (n = 6, C = 2.5 mu g L-1 of each cation) and detection limits were in the ranges of 3.4-4.1% and 0.38-0.42 mu g L-1, respectively. Finally, the developed method was successfully applied for the determination of Cd(II), Cu(II), and Pb (II) ions in different milk samples.