Journal
TALANTA
Volume 206, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.120250
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Funding
- Spanish Ministry of Economy and Competitiveness (MINECO) [CTQ-2016-77155-R]
- FEDER funds
- Conselleria d'Innovacio, Recerca I Turisme of the Govern de les Illes Balears
- Fons Social Europea [FPI/1867/2016]
- Thepsatri Rajabhat University
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The development of a simple and economical spectrophotometric system based on the use of a device created by 3D printing and the electronics necessary to control the intensity of the radiation source was described. The measurements are made with a low-cost digital webcam. The entire system is only powered through the USB outputs of a computer, which makes the portable and really practical system for the measurements in the field. This method was applied to determine iron (II) in waters using o-phenanthroline as chromogenic reagent giving a red complex, and also to hypochlorite determination using tetramethylbenzidine as the reagent providing a yellow color. The calibration curves were built using a mathematical algorithm making a RGB deconvolution. The intense of colors obtained from a webcam in each concentration of analyte had a relationship with the absorbance values. In order to confirm the accuracy and precision of this method, a traditional spectrophotometer was used for validation.
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