Journal
BIOSENSORS & BIOELECTRONICS
Volume 72, Issue -, Pages 230-236Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.005
Keywords
Anthrax; Surface-enhanced Raman scattering; Microfluidics; Solenoid; Poly-gamma-D-glutamic acid
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Funding
- National Research Foundation of Korea [K20904000004-14A0500-00410]
- Research Program from the Korea Centers for Disease Control and Prevention [2014-E45002-00]
- Agency for Chemical & Biological Detection Research Center (CBDRC)
- National Research Foundation of Korea [2009-00426] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We report the application of a fully automated surface-enhanced Raman scattering (SERS)-based solenoid-embedded microfluidic device to the quantitative and sensitive detection of anthrax biomarker poly-gamma-D-glutamic acid (PGA) in solution. Analysis is based on the competitive reaction between PGA and PGA-conjugated gold nanoparticles with anti-PGA-immobilized magnetic beads within a microfluidic environment. Magnetic immunocomplexes are trapped by yoke-type solenoids embedded within the device, and their SERS signals were directly measured and analyzed. To improve the acccuracy of measurement process, external standard values for PGA-free serum were also measured through use of a control channel. This additional measurement greatly improves the reliability of the assay by minimizing the influence of extraneous experimental variables. The limit of detection (LOD) of PGA in serum, determined by our SERS-based microfluidic sensor, is estimated to be 100 pg/mL. We believe that the defined method represents a valuable analytical tool for the detection of anthrax-related aqueous samples. (C) 2015 Elsevier B.V. All rights reserved.
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