Journal
BIOSENSORS & BIOELECTRONICS
Volume 64, Issue -, Pages 57-62Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.08.054
Keywords
CoFe layered double hydroxides; Carbon nanodots; Horseradish peroxidase; Hydrogen peroxide; Electrocatalysis
Categories
Funding
- National Natural Science Foundation of China [21075001, 20801001]
- Natural Science Foundation of Anhui Province [1408085QB27]
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Carbon nanodots and CoFe layered double hydroxide composites (C-Dots/LDHs) were prepared via simply mixing C-Dots and CoFe-LDHs. The as-prepared composites were used for the immobilization of horseradish peroxidase (HRP) on the glass carbon (GC) electrode. The electrochemical behavior of the HRP/C-DotsILDHs/GC electrode and its application as a H2O2 biosensor were investigated. The results indicated that HRP immobilized by C-Dots/LDHs retained the activity of enzyme and displayed quasi-reversible redox behavior and fast electron transfer with an electron transfer rate constant k(s) of 8.46 s(-1.) Under optimum experimental conditions, the HRP/C-Dots/LDHs/GC electrode displayed good electro-catalytic reduction activity and excellent analytic performance toward H2O2. The H2O2 biosensor showed a linear range of 0.1 -23.11 mu M (R-2=0.9942) with a calculated detection limit of 0.04 mu M (S/N=3). In addition, the biosensor exhibited high sensitivity, good selectivity, acceptable reproducibility and stability. The superior properties of this biosensor are attributed to the synergistic effect of HRP, C-Dots and CoFe-LDHs, which has been proved by investigating their electrochemical response to H2O2. Thus the C-Dots and LDHs composites provide a promising platform for the immobilization of redox enzymes and construction of sensitive biosensors. (C) 2014 Elsevier B.V. All rights reserved.
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