Journal
BIOSENSORS & BIOELECTRONICS
Volume 70, Issue -, Pages 5-14Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.03.006
Keywords
Microfluidic; Miniaturised ELISA; Smartphone readout; Fluorescence; Colorimetric; Point of care diagnostics
Categories
Funding
- Capillary Film Technology Ltd
- Loughborough University
- EPSRC [EP/L013983/1]
- EPSRC [EP/L013983/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/L013983/1] Funding Source: researchfish
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We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60 ng/ml with <7% precision in 13 min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08 ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22 min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology. (C) 2015 The Authors. Published by Elsevier B.V.
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