Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 229, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117988
Keywords
Fluorescent probe; Ratiometric; Formaldehyde; Imaging; Benzothiazole
Categories
Funding
- National Natural Science Foundation of China [21804085, 21605100, U1904195, U1404215]
Ask authors/readers for more resources
We presented a new benzothiazole-based fluorescent probe for ratiometric sensing of formaldehyde. Upon treatment with formaldehyde, the alkylamine-functionalized probe can be converted to its aldehyde analogue via the target-mediated 2-aza-Cope rearrangement, which led to significant shifts in both absorption (from 392 to 452 nm) and emission (from 492 to 552 nm) bands. The sensing mechanism was confirmed by HPLC, UV/Vis and fluorescence spectroscopy. The probe is capable of sensing formaldehyde under physiological conditions with high selectivity over potentially competing biological analytes. The probe also displayed sensitive ratiometric fluorescence response (up to 35.7 fold) for formaldehyde with a low limit detection of 0.58 mu M. Furthermore, the probe was successfully employed for ratiometric imaging of formaldehyde in living cells as well as in zebrafish. (C) 2019 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available