Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 224, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117403
Keywords
Fluorescent probe; Near-infrared; Glutathione (GSH); High-temperature stress; Living cells
Categories
Funding
- Science and Technology Developing Foundation of Jilin Province of China [20180201050YY, 201701011106JC]
- Industrial Innovation Funds of Jilin Province of China [2018C034-1]
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In this study, a water-soluble, near-infrared fluorescent probe (EQR-S) was designed for the measurement of the glutathione (GSH) concentration. Responses of different interfering substances to the developed probe were investigated, and the luminescence mechanism was examined by theoretical calculations. Results revealed that EQR-S can be applied for the rapid, sensitive determination of the GSH concentration with a detection limit of 69 nM. Based on the above advantages, EQR-S was successfully applied to investigate the fluctuation in the GSH concentration of living cells under high-temperature stress. (c) 2019 Elsevier B.V. All rights reserved.
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