15N tracing studies including plant N uptake processes provide new insights on gross N transformations in soil-plant systems

Most soil N transformations studies are carried out using soil incubations without plants, despite the fact that plant-soil interactions potentially influence soil N dynamics. In this study, gross N transformation rates were quantified using a subtropical acidic forest with and without plants (and under different soil storage conditions). The results showed that the gross rates of N mineralization in air-dried and rewetted soil significantly increased, while the gross rates of nitrification and immobilization decreased, compared with fresh soil. Soil storage for more than one month at 4 degrees C (typical refrigerated conditions) and room temperature (25 degrees C) did not affect the gross rates of soil N mineralization and immobilization but significantly inhibited heterotrophic nitrification rates. Moreover, plants grown in the soil significantly stimulated gross rates of N mineralization, autotrophic and heterotrophic nitrification, and NO3- immobilization. Plant NI-It uptake rates (3.74 mg N kg(-1) d(-1)) were 374 times greater than the NH4+ immobilization rate (0.01 mg N kg(-1) d(-1)). The competition for NHS' between plants and soil microorganisms led to strong feedback effects on soil N transformations. Based on our results we recommend to carry out N-15 tracing studies with plants to more realistically mimic field conditions. N-15 tracing techniques in combination with N-15-tracing models, such as Ntrace(ptant), provide a robust method to quantify soil N transformations and plant N uptake rates in plant-soil systems.