Journal
SMALL
Volume 16, Issue 3, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201906273
Keywords
3D culture; Alzheimer's disease; amyloid-beta; cognition repair; exosomes
Categories
Funding
- National Natural Science Foundation of China [31700831]
- Goverment of Jiangsu Province Foundation, China [BE2017665]
Ask authors/readers for more resources
Reducing amyloid-beta (A beta) accumulation could be a potential therapeutic approach for Alzheimer's disease (AD). Particular functional biomolecules in exosomes vested by the microenvironment in which the original cells resided can be transferred to recipient cells to improve pathological conditions. However, there are few reports addressing whether exosomes derived from cells cultured on scaffolds with varying dimension can reduce A beta deposition or ameliorate cognitive decline for AD therapy. Herein, both 3D graphene scaffold and 2D graphene film are used as the matrix for human umbilical cord mesenchymal stem cell culture, from which the supernatants are obtained to isolate exosomes. The levels of 195 kinds of miRNAs and proteins, including neprilysin, insulin-degrading enzyme and heat shock protein 70, in 3D-cultured exosomes (3D-Exo) are dramatically different from those obtained from 2D culture. Hence, 3D-Exo could up-regulate the expression of alpha-secretase and down-regulate the beta-secretase to reduce A beta production in both AD pathology cells and transgenic mice, through their special cargo. With rescuing A beta pathology, 3D-Exo exerts enhanced therapeutic effects on ameliorating the memory and cognitive deficits in AD mice. These findings provide a novel clinical application for scaffold materials and functional exosomes derived from stem cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available