Journal
BIOSENSORS & BIOELECTRONICS
Volume 72, Issue -, Pages 56-60Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.04.089
Keywords
Cardiac marker; Myoglobin; Aptamer; rGO/CNT nanohybrid; Direct electron transfer
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Funding
- INST PDF
- CSIR-JRF
- DST, Govt. of India
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We report a label free electrochemical detection of cardiac bio-marker myoglobin (Mb) on aptamer functionalized rGO/CNT nanostructured electrodes by measuring its direct electron transfer (DET). Configured as a highly responsive aptasensor, the newly developed biosensing platform exhibits synergistic effect of the nano-hybrid functional construct by combining good electrical properties and the facile chemical functionality of nanohybrid for the compatible bio-interface development. The specific anti-Mb aptamer was generated by five iterative SELEX (Systematic evolution of ligands by exponential enrichment) rounds, showing high senstivity (K-D similar to 65 pM). The aptamer functionalized rGO/CNT nanostructured electrodes demonstrated a significant increase in signal response with a detection limit of similar to 034 ng/mL in the dynamic response range between 1 ng/mL and 4 mu g/mL for Mb. The newly developed DET assay format presents a promising candidate in point-of-care diagnosis for routine screening of Mb in patient's samples. (C) 2015 Elsevier B.V. All rights reserved.
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