Journal
BIOSENSORS & BIOELECTRONICS
Volume 72, Issue -, Pages 197-204Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.05.017
Keywords
Gold nanoparticles (AuNPs); Localized surface plasmon resonance (LSPR); Rayleigh scattering; Beta amyloid (A beta); Apolipoprotein4 (ApoE4)
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Funding
- National Research Foundation of Korea (NRF) Grant - Korea government (Ministry of Science, ICT & Future Planning) [NRF-2013R1A2A1A01015644/2010-0027955/2012R1A2A1A01008085]
- University-Institute Cooperation Program
- Korea CCS R&D Centre Grant - Korea government (Ministry of Science, ICT & Future Planning) of the Republic of Korea [2014M1A8A1049278]
- Korea Institute of Energy Technology Evaluation and Planning
- Ministry of Trade, Industry & Energy of Korea [20122010200010-11-2-100]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20122010200010] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Beta amyloid (A beta) deposition is a pathological milestone of Alzheimer's disease (AD). This is facilitated by an isoform of Apolipoprotein E4 (ApoE4), which is a dominant risk factor for AD. However, current in vitro A beta aggregation assays were performed in extreme conditions not linked to physiological conditions, to understand the mechanism of A beta induced neurotoxicity. Here, we present a simple method for the ApoE4-mediated A beta aggregation at physiological conditions using single gold nanoparticle based on localized surface plasmon resonance (LSPR). It can be directly observed by dark-field microscope or even by the naked eye. Following LSPR principles, we used ApoE4 inducing A beta 42 self-assemblies on gold nanoparticles (AuNPs) surface via their surface charge interaction. Using physiologically mimic cerebrospinal fluid, we determined a detection limit of 1.5 pM for A beta 42 corresponding to the similar to 2.9 nm LSPR-peak shift under ApoE4. Interestingly, the result also shows that ApoE4 induces the aggregation of A beta 42 more specifically and rapidly than that of A beta 40. This is the first biomimetic platform for real-time detection of A beta aggregation, mimicking biological conditions, which can be used to investigate AD directly. (C) 2015 Elsevier B.V. All rights reserved.
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