Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 305, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.127346
Keywords
Droplet microfluidics; Magnetic beads; Extraction and redispersion; DNA size selection; NGS
Funding
- French National Research Agency (ANR) as part of the Investissements d'Avenir program [ANR10-NANO0207]
- ERC CellO [FP7-IDEAS-ERC-321107]
- Curie Institute International PhD fellowship
- Agence Nationale de la Recherche (Investissements d'Avenir program) [ANR-10-EQPX-03, ANR-10-INBS-09-08]
- Canceropole Ile-de-France
- SiRIC-Curie program - SiRIC Grant [INCa-DGOS-4654]
- French National Research Agency (ANR) as part of the Investissements d'Avenir program (Labex and Equipex IPGG)
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We present an innovative droplet microfluidic device based on integrated soft-magnetic structures allowing extraction, redispersion and depletion processes of magnetic particles. Following a numerically-assisted conception, the device was at first fabricated and experimentally characterized, showing reliable and efficient magnetic particles extraction with a low carryover of the initial matrix (< 3.5%), in a broad range of dispersed phase viscosities (between 1 and 15 mPa.s) and particles loads (between 0.1 mu g and 1.5 mu g). Its functionalities were successfully exploited to perform DNA size selection processes for NGS libraries preparation. The device achieved final concentrations and size distributions comparable with conventional methods, demonstrating compatibility with downstream sequencing and providing improvements in automation, throughput and workflow simplification. Finally, this device represents a universal solution for high throughput multi-steps magnetic solid phase extraction workflows in a wide range of applications, e.g. immunoassays, nucleic acids and proteins purification, and sample preparation.
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