Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 307, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.127643
Keywords
beta-Galactosidase; Fluorescent probe; Live cells; In vivo; Two-photon
Funding
- NSFC [21502067, 21672083, 21877048]
- Natural Science Foundation of Shandong Province, China [ZR2014BP001]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004, 160082101]
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beta-galactosidase plays an important role in early cancer diagnosis and is widely used in examine transcription and transfection efficiencies. In addition, abnormally accumulated beta-galactosidase activity has been widely accepted marker of cellular senescence. Therefore, it is necessary to develop an effective method to detect beta-galactosidase activity in biological systems. To achieve this goal, we have developed a fluorescent probe-by using Rho as a fluorophore and linking it to galactose via an ether bond for sensing beta-galactosidase. The probe TR-G with high selectivity and sensitivity has an emission wavelength above 600 nm, and further, cell and two-photon tissue imaging are performed and an excellent tissue penetration is presented. Thus, TR-G is able to detect beta-galactosidase in vivo and in vitro.
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