Journal
BIOSENSORS & BIOELECTRONICS
Volume 70, Issue -, Pages 246-253Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.03.035
Keywords
Au nano-clusters; 6-mercaptopurine; Fluorescence analysis; Fe3O4 nanoparticles
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Funding
- National Natural Science Foundation of China [NSFC-21065007, NSFC-21305061]
- Graduate Student Innovation Program of Nanchang University [YC2014-S042]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZA-201302, SKLF-ZZB-201303]
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A magnetic and fluorescent nano-composite was prepared. It comprised of a core of Fe3O4 nanoparticles (NPs), a silica shell and satellitic Au nano-clusters (AuNCs) capped with bovine serum albumin (BSA). This nano-composite has many desirable properties, e.g. magnetism, red emission, high water solubility, and high resistance to photo-bleaching. On addition of the analyte, 6-mercaptopurine (6-MP) or indeed other similar thiols, AuNCs formed aggregates because the existing cross-links within the Fe3O4 NP5@SiO2 and AuNC structure were broken in favor of the gold-thiol bonds. On suitable irradiation of such aggregates, red fluorescence was emitted at 613 nm. It decreased significantly as a function of the added 6-MP concentration, and the quenching ratio (F-0 - F) / F-0 was related linearly to the concentration of 6-MP in the range of 0.01 to 0.5 mu mol L-1. The detection limit was 0.004 mu mol L-1 (S/N=3). The method was strongly selective for 6-MP in the presence of oxidants, phenols, heavy-metal ions, and especially biothiols. (C) 2015 Elsevier B.V. All rights reserved.
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