Journal
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
Volume 45, Issue 6, Pages 656-661Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1068162019060141
Keywords
SELEX; combinatorial libraries of nucleic acids; solid-phase synthesis; high throughput sequencing
Funding
- Russian Scientific Foundation [16-14-10296]
- [AAAA-A17-117020210021-7]
- Russian Science Foundation [16-14-10296] Funding Source: Russian Science Foundation
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Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers with specified properties could be obtained from combinatorial libraries of nucleic acids by in vitro selection procedure. Here, we optimized conditions of chemical synthesis of DNA library on the automated ASM-800 DNA/RNA synthesizer. Enzymatic hydrolysis and high-throughput sequencing methods were used to analyze compositions of synthesized combinatorial libraries. The DNA library generated under the chosen conditions is characterized by the sufficiently uniform randomization and can be applied for the in vitro selection.
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